Poly (acrylic acid)/tricalcium phosphate nanoparticles scaffold enriched with exosomes for cell-free therapy in bone tissue engineering: An in vivo evaluation
نویسندگان
چکیده
Introduction: This study aimed to assess the potential of poly (acrylic acid)/tricalcium phosphate nanoparticles (PAA/triCaPNPs) scaffold in terms biocompatibility and osteoconductivity properties in-vivo evaluation as well investigate performance PAA/triCaPNPs (with or without exosomes derived from UC-MSCs) for bone regeneration rat critical-sized defect. Methods: was made acrylic acid (AA) monomer, N,N’-methylenebisacrylamide (MBAA), sodium bicarbonate (SBC), ammonium persulfate (APS) through freeze-drying method. For vivo evaluation, we randomly divided 24 rats into three groups. The calvarial defects were treated follows: (1) Control group: any treatment, (2) with only, (3) scaffold+exo enriched (1 μg/μl ,150 μg/per rat). Eight- 12-weeks post-surgery, half animals sacrificed examined micro-computerized tomography (µ-CT), histological staining, immunohistochemistry (IHC). Results: Quantitative analysis based on µ-CT scan images at 8 12 weeks post-implantation clearly indicated that healing rate filled exosome significantly higher than exosome. H&E Masson staining results revealed more new bone-like form developed group control Further, IHC osteocalcin CD31 confirmed could be associated osteogenesis angiogenesis concurrently. Conclusion: In present study, therapeutic a carrier human UC-MSC-derived achieve exosome-controlled release exosome-enriched effectively minify defect area improve model, such it an option exosome-based therapy.
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ژورنال
عنوان ژورنال: Bioimpacts
سال: 2023
ISSN: ['2228-5652', '2228-5660']
DOI: https://doi.org/10.34172/bi.2023.27510